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SRX4297675: GSM3221231: EtOH-rep2; Anopheles gambiae; RNA-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 16.6M spots, 4.4G bases, 1.8Gb downloads

Submitted by: NCBI (GEO)
Study: Influence of 20E on mosquito gene expression
PRJNA477857 • SRP151310 • All experiments • All runs
show Abstracthide Abstract
To examine the effects of 20E treatment on mosquito gene expression, treated mosquito cells were examined by RNA-seq to determine the influence of 20E treatment. Overall design: Mosquito cell lines were exposed to 10% ethanol (control) or 20-hydroxyecdysone (20E) in 10% ethanol. After exposure for 24 hrs, cells were harvested for RNA isolation. Samples were collected from four independent biological experiments.
Sample: EtOH-rep2
SAMN09484320 • SRS3460627 • All experiments • All runs
Organism: Anopheles gambiae
Library:
Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Following cell treatments, cells were incubated for 24 hours before control- and 20E-treated cell samples were isolated. RNA was isolated using TRIzol reagent and evaluated for quality using an Agilent 2100 Bioanalyzer. Samples were sent to the DNA Facility at Iowa State University for library construction and stranded mRNA-Seq. Gene expression analysis was performed the Genome Informatics Facility at Iowa State University. RNA libraries were prepared for sequencing using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM3221231
Links:
Runs: 1 run, 16.6M spots, 4.4G bases, 1.8Gb
Run# of Spots# of BasesSizePublished
SRR742685316,565,7784.4G1.8Gb2020-04-23

ID:
5785060

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